Technologies

What De-Extinction Means for the Living World

With much controversy swirling around Colossal’s proxy dire wolves, we spoke with the company’s CEO to find out how these animals were created and what so-called de-extinction technology could mean for endangered species.

Some people will tell you that the dire wolves are back, while others will tell you they are not. Colossal Biosciences kicked off an avalanche of media coverage, including both hype and harsh criticism, when the company said it brought back dire wolves, an extinct species given renewed popularity by its role in the Game of Thrones franchise, using gene-editing and cloning technology.

We will discuss the details of what Colossal has accomplished, but this technology also raises a bigger question with wider-reaching implications: What does de-extinction mean for the living world and endangered species fighting for survival?

Proxy dire wolves

Three animals — two males named Romulus and Remus and a female named Khaleesi — are at the center of the controversy.

Colossal created the wolf pups by taking DNA from an existing species, the gray wolf, and making 20 edits to 14 genes designed to produce traits associated with the dire wolf: size, the color of their coats and more. The embryos were implanted into large dogs and reportedly delivered by a planned cesarean section.

Much of the debate around this issue has centered on whether gene-edited gray wolves can be accurately called “dire wolves” and if their creation can truly be considered “de-extinction.”

“It’s never gonna be possible to bring something back that’s genetically identical to a species that used to be around, and also a species is more than just its DNA,” said Beth Shapiro, Colossal’s chief science officer. “It’s the DNA interacting with the ecosystem, which also isn’t present.”

The International Union for the Conservation of Nature defines de-extinction as “the process of creating an organism that resembles an extinct species,” with the critical caveat that the term itself is misleading. Thinking of de-extinct versions of animals as a proxy for the original animals, rather than a faithful reproduction, adds some clarity to the conversation.

How Colossal’s de-extinction works

To understand how it works, look at the first gene-edited species Colossal announced: woolly mice. This demonstrates the company’s ability to make several gene edits at once.

“It’s the same genes that evolved in elephants and evolved in mice, but it was variants in those genes we know are compatible with a healthy mouse,” Shapiro said.

Colossal has produced about 38 woolly mice, and more are on the way. The mice are also reproducing.

Colossal seeks to answer some of the questions with its wooly mice include whether the edits will affect the mice’s ability to thrive in colder climates and the long-term effects of CRISPR gene editing at multiple sites in the animal’s genome.

Animal welfare

Colossal says it prioritizes the creation of healthy animals over things like editing the maximum number of genes or adding in genes exactly as they existed in extinct species. “Every edit carries some risk,” Shapiro said. “We did AI modeling of 3D protein folding, turning genes up and down slightly rather than changing them in some dramatic way.”

Even with precautions, Shapiro acknowledged things don’t always go according to plan. Khaleesi, Colossal’s female proxy dire wolf, had a sister who died at about day 10 due to an infection. “It was confirmed not to be a result of our editing,” Shapiro said.

Endangered species

Along with the proxy dire wolves, Colossal says it has cloned four endangered red wolves. The US Fish & Wildlife Service has estimated that fewer than 20 red wolves are left in the wild and about 270 are in captivity.

Colossal’s red wolf clones were made using genes from what the company is calling “ghost wolves,” a population of what were believed to be coyotes living in western Louisiana and eastern Texas that were revealed to have a significant portion of red wolf DNA.

Colossal CEO Ben Lamm says the clones “increased genetic diversity of the captive breeding pool by up to 25%.”

Lamm also highlighted the company’s pledge to make much of its de-extinction technologies available for free, including genetic rescue (adding genetic diversity to small at-risk populations), biobanking (preservation of biological samples) and cryo-freezing (cold storage of biological samples).

What’s next?

Questions and concerns still loom, many of which have been laid out by the IUCN, the same organization that provided Colossal’s working definition of de-extinction and acknowledged it as misleading.

Among the potential disadvantages to de-extinction raised by the IUCN is a possible decrease in support for preventing extinctions. If the public misbelieves that extinction is reversible due to the popularity of de-extinction in the news, could that promote apathy about ongoing conservation efforts?

Risk to animals, including those created by gene-editing and cloning, surrogate animals and any potential future offspring or hybrid species (even re-extinction), is also a concern.

Then there are the challenges of potential rewilding, its effects on the ecosystem, the potential for invasiveness of proxy species in their new environment and the risk of disease.

This brings us back to where we began, with the trio of proxy dire wolves who will live out their lives on a 2,000-acre preserve in an undisclosed location.

Check out the video in this article to see Colossal’s proxy dire wolves in action and hear our interviews with the company’s CEO and chief science officer.

Technologies

Trump Maintains US‑Iran Negotiations Continue Amid Tehran’s Denials of Duplicity

President Trump insists that US‑Iran talks are ongoing despite Tehran’s denial of any negotiation plans, while warning that only a deal or total surrender will allow passage through the Strait of Hormuz. Conflicting statements from both sides have heightened uncertainty as the conflict enters its sixth month.

On Monday, President Donald Trump asserted that negotiations between the United States and Iran are still taking place, even after Tehran stated it has no intention of engaging in direct talks with Washington.

In a fiery Truth Social post, Trump labeled Iran’s leaders “unbelievably duplicitous,” claiming they are lying about ongoing peace talks “whether Iran wants to admit it or not.” He repeated his assertion that the United States completely controls the Strait of Hormuz, despite maritime traffic through the crucial route lingering at only a small fraction of pre‑conflict levels.

He wrote, “Nothing reaches Iran unless we allow it, and nothing will pass unless a deal—or total surrender—is achieved.”

Earlier that day, Iranian Foreign Ministry spokesperson Esmail Baghaei told reporters there is no imminent plan for U.S.–Iran negotiations, contradicting Trump’s earlier comment that talks would resume Monday afternoon. Baghaei added that Iran’s only current discussions are with Oman concerning the Strait of Hormuz.

The conflicting statements have heightened uncertainty over the peace‑talk process and the broader conflict, now in its sixth month.

Trump’s assertion about new negotiations came a day after he said on Truth Social that he had agreed to cancel a massive strike against Iran “subject to being able to rapidly make a DEAL.” He said in the same post that Iran and other Middle Eastern countries had asked him to hold off on that attack because “the perimeters of a deal has been agreed to.”

Trump has claimed dozens of times throughout the more‑than‑five‑month‑long war that a deal is at hand. No permanent deal has been signed, and a temporary ceasefire reached in June has fallen apart.

Trump has also repeatedly threatened to launch devastating strikes against Iran before backing off. After the latest example, oil prices on Monday fell and stocks surged.

BMI, a research unit of Fitch Solutions, said in a note Monday that a broader diplomatic understanding on reopening the Strait of Hormuz is still achievable this quarter, while raising the probability of its escalation scenario to 35% from 25%, citing mounting military, diplomatic and economic signs of rising U.S.-Iran tensions.

“Diplomatic progress is likely to be punctuated by periodic military flare-ups, while miscalculation by either side could trigger a renewed escalation,” BMI analysts wrote in a note. The firm said the key issue to watch is the future governance of the strait, as the Iran-Oman talks — potentially backed by Gulf states, China and the U.S. — point to efforts to build a post-conflict shipping framework.

Shipping risks persist even as diplomacy appears to be advancing. The United Kingdom Maritime Trade Operations Centre said it received a report of an incident 20 nautical miles (23 miles) northeast of Khasab, Oman — at the mouth of the strait — with a tanker’s master reporting an explosion in close proximity to the vessel at about 20:37 UTC Sunday (4:37 pm ET). The vessel and crew were safe and authorities are investigating, UKMTO said, advising ships to transit with caution.

The proposal Trump announced over the weekend calls for the U.S. and Iran to return to negotiations and continue ironing out some of the thorny issues that had derailed diplomatic efforts, according to The Associated Press, citing a regional official involved in the mediation efforts.

The official said the proposal also includes a reopening of the Hormuz Strait and halting attacks across the region, including by Iranian-backed militias in Iraq on the Arab Gulf countries and Jordan.

The U.S., for its part, will end its naval blockade on Iran and allow Tehran to export its oil, the official said, adding that no deal has been reached, although the mediation efforts remained underway.

Trump’s weekend reversal has lowered the temperature after days of escalating attacks across the Gulf. Kuwait said Saturday that Iranian forces launched a wave of drones within its airspace, with its military destroying multiple aircraft after Iran targeted critical infrastructure in the country’s north.

A parallel track with Muscat is also advancing. Iranian diplomats said Tehran was close to reaching a new arrangement with Oman to manage shipping through the Strait of Hormuz, a deal critical to preventing the war from escalating further, according to the Financial Times.

Iranian officials said negotiations over future management of the Hormuz Strait with Oman, which sits on the opposite shore of the waterway, are in their final stages. The agreed shipping route would be different from those used before, according to Iran’s Foreign Ministry spokesperson, Baghaei, adding that the new route was separate from the issue of the strait’s reopening or continued closure.

Technologies

Oil Prices Slide as Trump Halts Planned Iran Strike

Oil prices dropped after Trump cancelled a planned strike on Iran, with WTI down about 5% and Brent near 5% lower. He said the move followed requests from Tehran and regional allies for a pause while a deal shaping the Strait of Hormuz and Iran’s nuclear program is negotiated.

Oil prices fell on Monday after President Donald Trump announced he had cancelled a planned strike on Iran. WTI futures dropped roughly 5% to $80.34 a barrel, while Brent slipped about 4.7% to $83.77 a barrel.

Trump said early Sunday he called off the strike after Iran and several Middle Eastern nations asked him to hold off, noting that the outlines of a deal had been agreed upon. He added that the prospective accord would entail the immediate, full opening of the Strait of Hormuz and an end to Iran’s nuclear ambitions, according to his Truth Social post.

The president had been considering another round of strikes as diplomatic hopes waned since the conflict began on February 28. He said the U.S. and Iran would meet for talks on Monday, but Iran denied any scheduled negotiations with Washington, citing PressTV. Iran’s foreign‑ministry spokesperson Esmaeil Baghaei clarified that Tehran was only discussing shipping routes through the Strait of Hormuz with Oman. In a follow‑up Truth Social message, Trump insisted that, regardless of Iran’s acknowledgment, the United States is indeed discussing a solution to a long‑standing problem created by Iran.

Technologies

Oil Prices Drop as Trump Cancels Planned Attack on Iran

Oil prices fell sharply after President Trump announced the cancellation of a planned strike on Iran, citing a new deal that would open the Hormuz Strait and end Iran’s nuclear threat.

Oil prices fell sharply on Monday after President Donald Trump announced that he had called off a planned strike on Iran.

West Texas Intermediate futures, the U.S. benchmark, slipped roughly 5% to close at $80.34 per barrel, while Brent crude, the international benchmark, declined 4.7% to settle at $83.77 a barrel.

Trump made the announcement early Sunday, saying he had canceled the strike following requests from Tehran and other Middle Eastern countries.

In a Truth Social post, he wrote: “We have just been asked by Iran, and other Middle Eastern Countries, to hold off any attack in that the perimeters of a deal has been agreed to.”

The president indicated that the proposed agreement would include the immediate, complete, and total opening of the Hormuz Strait, as well as an end to Iran’s nuclear threat.

Trump had been weighing additional strikes amid diminishing prospects for a diplomatic resolution to the conflict that began on Feb. 28. He stated that the U.S. and Iran would hold negotiations on Monday.

Tehran denied that talks were planned with Washington, according to state news outlet PressTV.

Iran’s Foreign Ministry spokesman Esmaeil Baghaei said Tehran was only holding talks with Oman regarding the routes ships can use through the Strait of Hormuz.

In a subsequent Truth Social post, Trump added that whether “Iran wants to admit it or not, we are, in fact, talking of a solution to a problem that they have caused for decades.”