Technologies

Sharpest images of living bacteria reveal unexpected membrane structure

The images are so clear that scientists can see proteins on a bacterium’s outer membrane.

Using a microscopy technique that pulls back the curtain on the nanoscale world, researchers have generated the sharpest images of live bacteria ever taken. The high-resolution snaps reveal a patchwork of proteins that make up the outer membrane and, potentially, give scientists a new way to attack the organisms.

In a study published Monday in the journal Proceedings of the National Academy of Sciences, researchers from the US and UK provide images of the rod-shaped bacterium Escherichia Coli, which is commonly found in our gastrointestinal tracts and helps digest food. It’s a mostly harmless citizen of our guts, but certain E. Coli strains can cause severe illness. In recent years, scientists have shown that these strains are becoming more resistant to antibiotics.

The bacteria are, of course, incredibly tiny. About 150 to 300 individual bacteria could probably fit head-to-toe across the period at the end of this sentence. While regular microscopes can see the bacteria as clumps of rods scattered all across a glass slide, they don’t give us a good idea of the structure of a bacterium’s body and the outer membrane that protects it from our drugs.

«The outer membrane is a formidable barrier against antibiotics and is an important factor in making infectious bacteria resistant to medical treatment,» Bart Hoogenboom, a nanotechnologist at University College London and co-author on the paper, said in a press release. Hoogenboom and his team wanted to take a much closer look and turned to «atomic force microscopy» to study the membrane in greater detail.

Atomic Force Microscopy, or AFM, is a powerful technique to study objects that are just nanometers in length. (A nanometer is a millionth of a millimeter.) Hooked up to a computer, the device can study the surface of tiny, tiny objects by running a fine-tipped probe, just a few nanometers wide, across the surface. The probe moves along the object — whether it’s a microbe, a piece of bone or another nanomaterial — and can sense the undulations present. You can imagine it kind of like a turntable stylus, moving along the grooves of a vinyl record. Totally different process but similar concept.

When the team ran the AFM probe over the surface of a living E. Coli bacterium, they discovered it was a mosaic of proteins. Most areas of the outer membrane were covered in mostly immobile proteins, but there were also islands that lacked any protein at all and were instead full of sugary lipid molecules.

«This suggests that the barrier may not be equally hard to breach or stretch all over the bacterium, but may have stronger and weaker spots that can also be targeted by antibiotics,» Hoogenboom said.

Outer membranes are present on what’s known as «gram-negative» bacteria, like E coli, and not gram-positive bacteria, like Staphylococcus. The «gram» doesn’t refer to weight, but a specific test designed to quickly differentiate bacteria based on their cell walls. Notably, gram-negative bacteria have a more impenetrable cell wall, which makes it hard for antibiotics to get in and work to destroy the microbes.

Antibiotic resistance makes infectious diseases harder to treat and can turn common infections into life-threatening scourges. Many antibiotic resistant infections are picked up in hospitals, and the World Health Organization has consistently listed antibiotic resistance as a global health threat over the past few years because it has the potential to upend health care systems.

How the protein patchwork of the outer membrane might affect the strength of a bacteria’s protective covering or how it could help combat antibiotic resistance is not yet known. But with advanced microscopy techniques, scientists can shine a light on a previously unseen realm.

Technologies

Today’s NYT Connections Hints, Answers and Help for Jan. 16, #950

Here are some hints and the answers for the NYT Connections puzzle for Jan. 16 #950.

Looking for the most recent Connections answers? Click here for today’s Connections hints, as well as our daily answers and hints for The New York Times Mini Crossword, Wordle, Connections: Sports Edition and Strands puzzles.

Today’s NYT Connections puzzle has a very intriguing blue group. You’ll notice some band names, but can you locate what they have in common? Read on for clues and today’s Connections answers.

The Times has a Connections Bot, like the one for Wordle. Go there after you play to receive a numeric score and to have the program analyze your answers. Players who are registered with the Times Games section can now nerd out by following their progress, including the number of puzzles completed, win rate, number of times they nabbed a perfect score and their win streak.

Read more: Hints, Tips and Strategies to Help You Win at NYT Connections Every Time

Hints for today’s Connections groups

Here are four hints for the groupings in today’s Connections puzzle, ranked from the easiest yellow group to the tough (and sometimes bizarre) purple group.

Yellow group hint: Worn on heads.

Green group hint: Nighty-night.

Blue group hint: Bands with something alphabet-related in common.

Purple group hint: Like great.

Answers for today’s Connections groups

Yellow group: Kinds of hats.

Green group: Components of a kid’s bedtime routine.

Blue group: Musical acts with «A» as the only vowel.

Purple group: Grand ____.

Read more: Wordle Cheat Sheet: Here Are the Most Popular Letters Used in English Words

What are today’s Connections answers?

The yellow words in today’s Connections

The theme is kinds of hats. The four answers are derby, fedora, Panama and porkpie.

The green words in today’s Connections

The theme is components of a kid’s bedtime routine. The four answers are bath, brushing, pajamas and story.

The blue words in today’s Connections

The theme is musical acts with «A» as the only vowel. The four answers are Alabama, Bananarama, Kansas and Santana.

The purple words in today’s Connections

The theme is grand ____. The four answers are Bahama, Canyon, piano and slam.

Don’t miss any of our unbiased tech content and lab-based reviews. Add CNET as a preferred Google source.

Quick tips for Connections

#1: Say the clue words out loud, pausing before and after each. That helps you hear the words in the context of a phrase. The Connections editors love to group words together that are used in similar phrasing, like ____ Up.

#2: Don’t go for the obvious grouping. These editors are smart. Once, they offered SPONGE, BOB, SQUARE and PANTS in the same puzzle. None of those words were in the same category. If you like, hit the «shuffle» button to give yourself a different perspective on the words.

#3: Break down any compound words and look for similarities. «Rushmore» was once in a puzzle where the connection was that each word started with the name of a rock band.

Technologies

Today’s NYT Connections: Sports Edition Hints and Answers for Jan. 16, #480

Here are hints and the answers for the NYT Connections: Sports Edition puzzle for Jan. 16, No. 480.

Looking for the most recent regular Connections answers? Click here for today’s Connections hints, as well as our daily answers and hints for The New York Times Mini Crossword, Wordle and Strands puzzles.

Today’s Connections: Sports Edition is a tough one, with some odd words and bizarre categories. If you’re struggling with it but still want to solve it, read on for hints and the answers.

Connections: Sports Edition is published by The Athletic, the subscription-based sports journalism site owned by The Times. It doesn’t appear in the NYT Games app, but it does in The Athletic’s own app. Or you can play it for free online.

Read more: NYT Connections: Sports Edition Puzzle Comes Out of Beta

Hints for today’s Connections: Sports Edition groups

Here are four hints for the groupings in today’s Connections: Sports Edition puzzle, ranked from the easiest yellow group to the tough (and sometimes bizarre) purple group.

Yellow group hint: Don’t get hurt!

Green group hint: Exaggerated language.

Blue group hint: Ouch!

Purple group hint: Not northern.

Answers for today’s Connections: Sports Edition groups

Yellow group: Protective gear.

Green group: Hype.

Blue group: Sharp things.

Purple group: «Southern ____» schools.

Read more: Wordle Cheat Sheet: Here Are the Most Popular Letters Used in English Words

What are today’s Connections: Sports Edition answers?

The yellow words in today’s Connections

The theme is protective gear. The four answers are gloves, helmet, mouth guard and shoulder pads.

The green words in today’s Connections

The theme is hype. The four answers are anticipation, ballyhoo, buildup and buzz.

The blue words in today’s Connections

The theme is sharp things. The four answers are dart, hairpin turn, ice skate and javelin.

The purple words in today’s Connections

The theme is «Southern ____» schools. The four answers are California, Illinois, Methodist and Miss.

Don’t miss any of our unbiased tech content and lab-based reviews. Add CNET as a preferred Google source.

Quick tips for Connections: Sports Edition

#1: Don’t grab for the easiest group. For each word, think about other sports categories it might fit in – is this a word that can be used in football, or to describe scoring options?

#2: Second meanings are important. The puzzle loves to use last names and even college names that mean other things, to fool you into thinking they are words, not names.

#3: And the opposite is also true. Words like HURTS might seem like a regular word, but it’s also the last name of at least one pro athlete.

Technologies

Today’s NYT Mini Crossword Answers for Friday, Jan. 16

Here are the answers for The New York Times Mini Crossword for Jan. 16.

Looking for the most recent Mini Crossword answer? Click here for today’s Mini Crossword hints, as well as our daily answers and hints for The New York Times Wordle, Strands, Connections and Connections: Sports Edition puzzles.

Need some help with today’s Mini Crossword? I thought it was exceptionally tough today, and was convinced 1-Across was «MAD» Max. (Spoiler, it’s not.) I also figured 1-Down was BOOZE, which did not work with 1-Across, and was wrong either way. Read on for all the answers. And if you could use some hints and guidance for daily solving, check out our Mini Crossword tips.

If you’re looking for today’s Wordle, Connections, Connections: Sports Edition and Strands answers, you can visit CNET’s NYT puzzle hints page.

Read more: Tips and Tricks for Solving The New York Times Mini Crossword

Let’s get to those Mini Crossword clues and answers.