Technologies

Sharpest images of living bacteria reveal unexpected membrane structure

The images are so clear that scientists can see proteins on a bacterium’s outer membrane.

Using a microscopy technique that pulls back the curtain on the nanoscale world, researchers have generated the sharpest images of live bacteria ever taken. The high-resolution snaps reveal a patchwork of proteins that make up the outer membrane and, potentially, give scientists a new way to attack the organisms.

In a study published Monday in the journal Proceedings of the National Academy of Sciences, researchers from the US and UK provide images of the rod-shaped bacterium Escherichia Coli, which is commonly found in our gastrointestinal tracts and helps digest food. It’s a mostly harmless citizen of our guts, but certain E. Coli strains can cause severe illness. In recent years, scientists have shown that these strains are becoming more resistant to antibiotics.

The bacteria are, of course, incredibly tiny. About 150 to 300 individual bacteria could probably fit head-to-toe across the period at the end of this sentence. While regular microscopes can see the bacteria as clumps of rods scattered all across a glass slide, they don’t give us a good idea of the structure of a bacterium’s body and the outer membrane that protects it from our drugs.

«The outer membrane is a formidable barrier against antibiotics and is an important factor in making infectious bacteria resistant to medical treatment,» Bart Hoogenboom, a nanotechnologist at University College London and co-author on the paper, said in a press release. Hoogenboom and his team wanted to take a much closer look and turned to «atomic force microscopy» to study the membrane in greater detail.

Atomic Force Microscopy, or AFM, is a powerful technique to study objects that are just nanometers in length. (A nanometer is a millionth of a millimeter.) Hooked up to a computer, the device can study the surface of tiny, tiny objects by running a fine-tipped probe, just a few nanometers wide, across the surface. The probe moves along the object — whether it’s a microbe, a piece of bone or another nanomaterial — and can sense the undulations present. You can imagine it kind of like a turntable stylus, moving along the grooves of a vinyl record. Totally different process but similar concept.

When the team ran the AFM probe over the surface of a living E. Coli bacterium, they discovered it was a mosaic of proteins. Most areas of the outer membrane were covered in mostly immobile proteins, but there were also islands that lacked any protein at all and were instead full of sugary lipid molecules.

«This suggests that the barrier may not be equally hard to breach or stretch all over the bacterium, but may have stronger and weaker spots that can also be targeted by antibiotics,» Hoogenboom said.

Outer membranes are present on what’s known as «gram-negative» bacteria, like E coli, and not gram-positive bacteria, like Staphylococcus. The «gram» doesn’t refer to weight, but a specific test designed to quickly differentiate bacteria based on their cell walls. Notably, gram-negative bacteria have a more impenetrable cell wall, which makes it hard for antibiotics to get in and work to destroy the microbes.

Antibiotic resistance makes infectious diseases harder to treat and can turn common infections into life-threatening scourges. Many antibiotic resistant infections are picked up in hospitals, and the World Health Organization has consistently listed antibiotic resistance as a global health threat over the past few years because it has the potential to upend health care systems.

How the protein patchwork of the outer membrane might affect the strength of a bacteria’s protective covering or how it could help combat antibiotic resistance is not yet known. But with advanced microscopy techniques, scientists can shine a light on a previously unseen realm.

Technologies

You’ll Soon Be Able to Buy Walmart Products Through ChatGPT

OpenAI’s chatbot already connects to Etsy and Shopify. Now you can buy bananas too.

OpenAI and Walmart will soon offer shopping via AI through ChatGPT, the retail giant said in a press release on Tuesday.

While using ChatGPT’s Instant Checkout feature, customers can buy groceries, electronics or other essentials within the chatbot interface.

Walmart has its own AI assistant in its app named Sparky. With Sparky, customers can ask questions about products and get summaries of reviews to find the best item.

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«For many years now, e-commerce shopping experiences have consisted of a search bar and a long list of item responses. That is about to change,» Walmart CEO Doug McMillon said in a statement. «There is a native AI experience coming that is multi-media, personalized and contextual. We are running towards that more enjoyable and convenient future with Sparky and through partnerships including this important step with OpenAI.»

When asked for comment, Walmart referred to its press release. Walmart also said it wouldn’t discuss the financial terms of the agreement at this time.

«We’re excited to partner with Walmart to make everyday purchases a little simpler. It’s just one way AI will help people every day under our work together,» OpenAI CEO Sam Altman said in a press release.

OpenAI referred to Walmart’s press release when asked for comment.

The latest deal with Walmart comes as OpenAI tries to make ChatGPT an all-in-one shopping experience. AI chatbots are increasingly being used as vehicles for online shopping. They can synthesize reviews from across the internet and give people direct answers to shopping questions. Already, ChatGPT connects with Etsy and Shopify with its Instant Checkout feature, allowing people to buy directly. OpenAI also added more shopping features in ChatGPT Search earlier this year.

(Disclosure: Ziff Davis, CNET’s parent company, in April filed a lawsuit against OpenAI, alleging it infringed Ziff Davis copyrights in training and operating its AI systems.)

Technologies

OpenAI Will Loosen ChatGPT’s Mental Health Guardrails and Allow Erotica for Adult Users

Sam Altman said the company will ease limits for adults after rolling out age verification.

ChatGPT is treading cautiously right now, but the chatbot may become more risqué by the end of the year.

In recent weeks, the generative AI chatbot has been operating under somewhat stringent limitations, as OpenAI tried to address concerns that it was not handling sensitive mental health issues well. But CEO Sam Altman said in a post on X Tuesday that the company would ease some of those restrictions because it’s «been able to mitigate the serious mental health issues.»

Though Altman didn’t elaborate on what tools are being used to address the problem, OpenAI recently announced new parental controls in ChatGPT.

CNET reached out to OpenAI for details, but the company did not immediately respond to a request for comment. (Disclosure: Ziff Davis, CNET’s parent company, in April filed a lawsuit against OpenAI, alleging it infringed Ziff Davis copyrights in training and operating its AI systems.)

Other changes are also expected. Altman said the company could allow «erotica» for verified adult users as it implements an «age-gating» system, or age-restricted content, in December. The mature content is part of the company’s «treat adult users like adults» principle, Altman said.

Altman’s post also announced a new version of ChatGPT in the next few weeks, with a personality that behaves more like the company’s GPT-4o model. Chatbot users had complained after the company replaced 4o with the impersonal GPT-5 earlier this year, saying the new version lacked the engaging and fun personality of previous chatbot models.

«If you want your ChatGPT to respond in a very human-like way, or use a ton of emoji, or act like a friend, ChatGPT should do it (but only if you want it, not because we are usage-maxxing),» Altman wrote.

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After OpenAI was sued by parents who alleged ChatGPT contributed to their teen son’s suicide, the company imposed an array of new restrictions and changes, including parental controls, alerts for risky behavior and a teen-friendly version of the chatbot. In the summer, OpenAI implemented break reminders that encourage people to occasionally stop chatting with the bot.

On Tuesday, the company also announced the creation of a council of experts on AI and well-being, including some with expertise in psychology and human behavior.

This comes as lawmakers and regulators are ringing the alarm on the risks AI tools pose to people, especially children. On Monday, California Governor Gavin Newsom signed new restrictions on AI companion chatbots into law. Last month, the Federal Trade Commission launched an investigation into several AI companies, including OpenAI.

Technologies

Today’s NYT Strands Hints, Answers and Help for Oct. 15 #591

Here are hints — and the answers — for the NYT Strands puzzle for Oct. 15, No. 591.

Looking for the most recent Strands answer? Click here for our daily Strands hints, as well as our daily answers and hints for The New York Times Mini Crossword, Wordle, Connections and Connections: Sports Edition puzzles.

Today’s NYT Strands puzzle is a fun one, once you understand the theme. Some of the answers are a bit tough to unscramble, so if you need hints and answers, read on.

I go into depth about the rules for Strands in this story.

If you’re looking for today’s Wordle, Connections and Mini Crossword answers, you can visit CNET’s NYT puzzle hints page.

Read more: NYT Connections Turns 1: These Are the 5 Toughest Puzzles So Far

Hint for today’s Strands puzzle

Today’s Strands theme is: Going up?

If that doesn’t help you, here’s a clue: Not an escalator, but…

Clue words to unlock in-game hints

Your goal is to find hidden words that fit the puzzle’s theme. If you’re stuck, find any words you can. Every time you find three words of four letters or more, Strands will reveal one of the theme words. These are the words I used to get those hints but any words of four or more letters that you find will work:

ROTATE, LOBE, NOPE, RATS, STAR, SAME, LOSE, VOTE, BUTTE, SAMS, BAMS

Answers for today’s Strands puzzle

These are the answers that tie into the theme. The goal of the puzzle is to find them all, including the spangram, a theme word that reaches from one side of the puzzle to the other. When you have all of them (I originally thought there were always eight but learned that the number can vary), every letter on the board will be used. Here are the nonspangram answers:

ALARM, OPEN, CLOSE, LOBBY, GROUND, BASEMENT

Today’s Strands spangram

Today’s Strands spangram is ELEVATORBUTTONS. To find it, look for the E that’s three letters to the right on the bottom row, and wind straight up, and then straight down.