Technologies

Sharpest images of living bacteria reveal unexpected membrane structure

The images are so clear that scientists can see proteins on a bacterium’s outer membrane.

Using a microscopy technique that pulls back the curtain on the nanoscale world, researchers have generated the sharpest images of live bacteria ever taken. The high-resolution snaps reveal a patchwork of proteins that make up the outer membrane and, potentially, give scientists a new way to attack the organisms.

In a study published Monday in the journal Proceedings of the National Academy of Sciences, researchers from the US and UK provide images of the rod-shaped bacterium Escherichia Coli, which is commonly found in our gastrointestinal tracts and helps digest food. It’s a mostly harmless citizen of our guts, but certain E. Coli strains can cause severe illness. In recent years, scientists have shown that these strains are becoming more resistant to antibiotics.

The bacteria are, of course, incredibly tiny. About 150 to 300 individual bacteria could probably fit head-to-toe across the period at the end of this sentence. While regular microscopes can see the bacteria as clumps of rods scattered all across a glass slide, they don’t give us a good idea of the structure of a bacterium’s body and the outer membrane that protects it from our drugs.

«The outer membrane is a formidable barrier against antibiotics and is an important factor in making infectious bacteria resistant to medical treatment,» Bart Hoogenboom, a nanotechnologist at University College London and co-author on the paper, said in a press release. Hoogenboom and his team wanted to take a much closer look and turned to «atomic force microscopy» to study the membrane in greater detail.

Atomic Force Microscopy, or AFM, is a powerful technique to study objects that are just nanometers in length. (A nanometer is a millionth of a millimeter.) Hooked up to a computer, the device can study the surface of tiny, tiny objects by running a fine-tipped probe, just a few nanometers wide, across the surface. The probe moves along the object — whether it’s a microbe, a piece of bone or another nanomaterial — and can sense the undulations present. You can imagine it kind of like a turntable stylus, moving along the grooves of a vinyl record. Totally different process but similar concept.

When the team ran the AFM probe over the surface of a living E. Coli bacterium, they discovered it was a mosaic of proteins. Most areas of the outer membrane were covered in mostly immobile proteins, but there were also islands that lacked any protein at all and were instead full of sugary lipid molecules.

«This suggests that the barrier may not be equally hard to breach or stretch all over the bacterium, but may have stronger and weaker spots that can also be targeted by antibiotics,» Hoogenboom said.

Outer membranes are present on what’s known as «gram-negative» bacteria, like E coli, and not gram-positive bacteria, like Staphylococcus. The «gram» doesn’t refer to weight, but a specific test designed to quickly differentiate bacteria based on their cell walls. Notably, gram-negative bacteria have a more impenetrable cell wall, which makes it hard for antibiotics to get in and work to destroy the microbes.

Antibiotic resistance makes infectious diseases harder to treat and can turn common infections into life-threatening scourges. Many antibiotic resistant infections are picked up in hospitals, and the World Health Organization has consistently listed antibiotic resistance as a global health threat over the past few years because it has the potential to upend health care systems.

How the protein patchwork of the outer membrane might affect the strength of a bacteria’s protective covering or how it could help combat antibiotic resistance is not yet known. But with advanced microscopy techniques, scientists can shine a light on a previously unseen realm.

Technologies

Harvard Business Review Study Finds ‘AI Brain Fry’ Is Leaving Workers Mentally Fatigued

Study participants reported increased mental fatigue while using AI tools, but less burnout overall.

Workers who excessively use AI agents and tools at work are at increased risk of mental fatigue, according to a recent Harvard Business Review study. In certain industries, more than 25% of hired professionals report increased mental strain due to their role in AI oversight — though these professionals also generally experienced less burnout than peers who aren’t using AI.

This phenomenon — which the researchers refer to as «AI brain fry» — is described as a «‘buzzing’ feeling or a mental fog» that caused study participants to develop headaches and difficulty focusing and making decisions. Individuals pointed to being overwhelmed by large amounts of information and to frequent task switching as the reasons for these feelings.

Studied individuals experienced more brain fry when they utilized AI agents to manage a workload beyond their own cognitive capacity. When participants used AI to replace mundane, repetitive tasks, managing the growing number of tools led to increased mental fatigue.

Crucially, the study found that fewer individuals who used these AI agents reported workplace burnout.

The researchers predict that this is because burnout testing assesses emotional and physical distress. In contrast, they report, acute mental fatigue «is caused by marshalling attention, working memory and executive control beyond the limited capacity of these systems.»

These are the processes that are taxed when study participants use multiple AI tools in their workflow, according to the researchers.

The Harvard study identifies several business costs incurred by workers suffering from AI brain fry. The foremost consequence is that these individuals may end up making lower-quality decisions. «Workers in [the] study who endorsed AI brain fry experience 33% more decision fatigue than those who did not,» the study reports. Workers who report AI brain fry were also more likely to self-report making both minor and major errors at their jobs.

Another recent Harvard Business Review study similarly found that employees who use AI tools «worked at a faster pace, took on a broader scope of tasks and extended work into more hours of the day,» but warned that «workload creep can in turn lead to cognitive fatigue, burnout and weakened decision-making.»

Technologies

Today’s NYT Connections Hints, Answers and Help for March 11, #1004

Here are some hints and the answers for the NYT Connections puzzle for March 11, No. 1,004.

Looking for the most recent Connections answers? Click here for today’s Connections hints, as well as our daily answers and hints for The New York Times Mini Crossword, Wordle, Connections: Sports Edition and Strands puzzles.

Once I spotted «ice cream» and «traffic» in today’s NYT Connections puzzle, I had the blue category all but filled in. But that purple category was even more bizarre than usual. Read on for clues and today’s Connections answers.

The Times has a Connections Bot, like the one for Wordle. Go there after you play to receive a numeric score and to have the program analyze your answers. Players who are registered with the Times Games section can now nerd out by following their progress, including the number of puzzles completed, win rate, number of times they nabbed a perfect score and their win streak.

Read more: Hints, Tips and Strategies to Help You Win at NYT Connections Every Time

Hints for today’s Connections groups

Here are four hints for the groupings in today’s Connections puzzle, ranked from the easiest yellow group to the tough (and sometimes bizarre) purple group.

Yellow group hint: Bring that back!

Green group hint: Fancy ____.

Blue group hint: Think of a certain shape.

Purple group hint: Sounds like…

Answers for today’s Connections groups

Yellow group: Steal.

Green group: Make nicer, with «up.»

Blue group: Kinds of cones.

Purple group: Pronoun homophones.

Read more: Wordle Cheat Sheet: Here Are the Most Popular Letters Used in English Words

What are today’s Connections answers?

The yellow words in today’s Connections

The theme is steal. The four answers are lift, palm, pinch and pocket.

The green words in today’s Connections

The theme is make nicer, with «up.» The four answers are dress, jazz, spiff and spruce.

The blue words in today’s Connections

The theme is kinds of cones. The four answers are ice cream, pine, snow and traffic.

The purple words in today’s Connections

The theme is pronoun homophones. The four answers are hee, mi, oui and yew.

Technologies

Today’s NYT Connections: Sports Edition Hints and Answers for March 11, #534

Here are hints and the answers for the NYT Connections: Sports Edition puzzle No. 534 for Wednesday, March 11.

Looking for the most recent regular Connections answers? Click here for today’s Connections hints, as well as our daily answers and hints for The New York Times Mini Crossword, Wordle and Strands puzzles.

Today’s Connections: Sports Edition features a real mix of categories, but the yellow one came easily to this Seahawks fan. If you’re struggling with today’s puzzle but still want to solve it, read on for hints and the answers.

Connections: Sports Edition is published by The Athletic, the subscription-based sports journalism site owned by The Times. It doesn’t appear in the NYT Games app, but it does in The Athletic’s own app. Or you can play it for free online.

Read more: NYT Connections: Sports Edition Puzzle Comes Out of Beta

Hints for today’s Connections: Sports Edition groups

Here are four hints for the groupings in today’s Connections: Sports Edition puzzle, ranked from the easiest yellow group to the tough (and sometimes bizarre) purple group.

Yellow group hint: Super Bowl champs’ division.

Green group hint: Baseball stats.

Blue group hint: Stars on ice.

Purple group hint: You wear it around your waist.