Technologies

2 Million-Year-Old DNA, the Oldest Ever Recovered, Opens Window to the Past

The prehistoric forest of northern Greenland was home to mastodons, reindeer, hares and an abundance of plant life.

As early as 2006, Eske Willerslev and members of his lab ventured into northern Greenland with a drill, extracting cores of sediment from the Kap København Formation. They were hunting for environmental DNA, or eDNA, in their cores — puzzle pieces that could help paint a picture of the plants and animals present in the region 2 million years ago.

But for the longest time, they came up empty-handed. «Every time we had improvements in terms of DNA extraction or sequencing technology, we’d revisit these samples,» Willerslev, an evolutionary geneticist at the University of Cambridge, said in a press briefing on Tuesday.

No matter what, the researchers failed to get what they were looking for. The run of bad luck saw members of the lab turn to the occult for an explanation; they named their troubles «the curse of the Kap København Formation.»

But with steady improvements in DNA extraction and sequencing technologies, the curse was finally broken.

On Wednesday, the team published the results of their 16-year pursuit of ancient DNA in the journal Nature. They were able to sequence eDNA from 41 sediment samples, collected in 2006, 2012 and 2016 from the Kap København Formation and undisturbed by humans for 2 million years. Their analyses revealed that a lush forest replete with reindeer, hares, mastodons and a wide variety of flora once stood in what is now a dull, gray polar desert.

Willerslev, a pioneering geneticist who has previously recovered eDNA from ice cores and shown it could survive in glaciers, noted that the «breakthrough» relied on expertise, advances in genetic sequencing techniques and bioinformatics.

History in the soil

DNA, which carries the instructions for life, is not a particularly sturdy molecule. The bonds that hold it together are weak and, over time, they break down.

This is why, even though we have an abundance of dinosaur fossils, we don’t have any dinosaur DNA. The beasts died out 66 million years ago, and the DNA would simply not survive that long.

When DNA degrades, the once-long strands of information break apart into smaller and smaller pieces. It becomes almost impossible to piece these fragments back together in the right configuration, especially if they are mixed in with a lot of other DNA from the environment.

Think of DNA like a book. Let’s say Alice in Wonderland. If you have the whole book, you can understand the story. But if you’re missing a few pages, you might not understand where the White Rabbit came from or why Alice ended up at a tea party with the Mad Hatter. If you’re missing lots of pages, you probably can’t even tell what the story was to begin with. Alice? Who’s that? And why is she 10 feet tall?

That’s the problem working with ancient DNA. You might be able to retrieve small fragments of DNA but it is generally too fragmented to be able to tell where it came from — and certainly too fragmented to understand where it came from.

But under certain circumstances, DNA fragments can survive deep time.

«The ‘survival time’ of DNA in the environment is incredibly variable and strongly dependent on the environment itself,» notes Michael Knapp, an ecologist and geneticist at Otago University in New Zealand.

Previously, the oldest DNA ever recovered came from mammoth fossils found in the Siberian permafrost. In a Nature paper in 2021, researchers showed that DNA from the mammoth teeth was, potentially, about 1.6 million years old. The DNA recovered was broken up into small fragments but they weren’t degraded so much they couldn’t be pieced back together. The cold temperature of the permafrost certainly helped with this.

It’s a similar story in the new study.

Willerslev and his collaborators postulate that the long survival time of the DNA in their sediment cores was possible for two reasons. The first is the constant cold temperature of the polar desert. The second is the way the DNA is bound to minerals in the cores, preventing degradation over longer time scales. The idea is that these mineral surfaces prevent enzymes from breaking down the DNA.

Karina Sand, a geochemist at the University of Copenhagen and co-author on the paper, explained that one of the technological leaps that enabled this feat was extracting DNA from clay and quartz minerals. The latter provided an abundance of DNA, but the former was harder to extract good DNA from. Fortunately, that leaves the door open for even older DNA extraction.

«If we can get better at extracting the DNA from the clay minerals, then we think we can go further back in time with DNA,» she said.

The research team was able to extract DNA from the sediment cores and begin to read the surviving fragments. These fragments were then compared to a database of genomes (complete DNA sequences) of modern plants and animals, looking for DNA matches. Over time, they were able to fill the blank pages of history, demonstrating the thriving ecosystem of ancient Greenland.

The ancient forest of Greenland

Two million years ago, Greenland was a different place.

«The Kap København ecosystem, which has no present-day equivalent, existed at considerably higher temperatures than we have today,» noted Mikkel Pederson, a geneticist at the Lundbeck Foundation GeoGenetics Centre, in a press release.

In northern Greenland, average temperatures during this time were likely more than 11 degrees Celsius (around 20 degrees Fahrenheit) higher than they are today. Previous studies at Kap København have shown evidence it was home to a boreal forest, but the eDNA extracted and analyzed in the new study provides a complete reimagining of the area, adding in megafauna and a wide variety of plant life.

The headline mammal DNA found in the cores is undoubtedly the mastodon — which is having a bit of a moment thanks to social media. Some of the eDNA found matched to the Elephantidae family, which includes elephants, mammoths and mastodons. It seems mastodons may have roamed Greenland 2 million years ago, though the researchers note the evidence isn’t extremely strong and is based on relatively weak DNA matches.

The team also found DNA related to reindeer, hares and rabbits, and the subfamily of animals that includes lemmings, voles and muskrats. Notably absent, however, is DNA from carnivores. The researchers suggest this is because of their comparably small biomass in relation to the herbivores. «It’s basically a numbers game,» Willerslev said.

One of the more intriguing DNA finds is of the Atlantic horseshoe crab. The species is no longer found at such northern latitudes, and the authors suggest this may mean Kap København experienced warmer sea surface temperatures 2 million years ago. Previous research has suggested the sea surface was warmer at higher latitudes, and the discovery of horseshoe crab DNA lends further support to this hypothesis.

Warmer temperatures are key. Multiple authors on the paper have reiterated the importance of understanding an ecosystem like this, given the effects of global warming. Two million years ago, the climate was changing and the eDNA shows that Arctic species were living with species that loved much warmer climes. This helps scientists to get an understanding of how nature was adapting to those changes and, within the DNA signatures, there may be clues to ways we could help modern-day fauna and flora survive extreme climatic swings.

One of the significant limitations of studying eDNA is that scientists have to postulate about the kinds of species that were living at the time. Knapp notes closely related ancient species might give you a DNA match but this is «somewhat inaccurate» — it provides an approximation of what existed. We may only be able to assign the DNA at a family or order level, so we can’t know exactly what roamed the boreal forest of Greenland 2 million years ago.

Even so, the recovery of DNA this old opens a new window to the prehistoric Earth, a pathway for scientists and researchers to probe the ecosystems that existed long before humans were around. The team will head to northern Canada to extract cores next year and hope to go even further back in time.

The extraction method may even lend itself to finding DNA in more humid climates across the world, like in Africa and Australia.

«If we can begin to explore ancient DNA in clay grains from Africa, we may be able to gather ground-breaking information about the origin of many different species —perhaps even new knowledge about the first humans and their ancestors,» Willerslev said in a statement.

«The possibilities are endless.»

Technologies

We Played Nintendo Switch 2: Mario, Donkey Kong, Mouse, Camera and a Lot More

Technologies

Nintendo Switch 2: Every Reveal About the Console, New Games, Price, Release Date

The $450 console launches June 5, with Mario Kart World the highlight of its launch day game lineup.

The Nintendo Switch 2 console’s biggest reveal yet arrived Wednesday as part of the company’s latest Nintendo Direct event. This teed up a day of Switch 2 reveals that include its June 5 release date, a $450 price, its specs and its initial game lineup that extends from launch into 2026.

The console will be further buoyed by having Mario Kart World as a launch title, which will go on sale in either a $500 bundle with the Switch 2 or on its own for $80 — making the open-world racing game one of Nintendo’s most expensive yet. Other games announced, such as Donkey Kong Bananza, are being priced around $70, which matches the cost of 2023’s The Legend of Zelda: Tears of the Kingdom. That Zelda game and Breath of the Wild are among the original Switch titles that will get enhanced Nintendo Switch 2 Edition upgrades, a premium upgrade allowing players to get new features, modes and graphical enhancements that take advantage of the newer system’s capabilities.

Here are the top highlights from Nintendo’s console event, all of the games we’ve heard about so far (including new titles, Nintendo Switch 2 Editions and GameCube games coming to Nintendo Switch Online) and all of our coverage so far. You can also check out our Nintendo Switch 2 live blog for even more updates about the Switch 2 as we learn them.

Nintendo Switch 2 console

The Nintendo Switch 2’s specs saw a noticeable technical bump over the Switch. The Nintendo Switch 2 has a 7.9-inch, 1080p resolution LCD that supports a 120Hz refresh rate — matching what we now see on most Android phones. When the Switch 2 is docked, compatible games can run in 4K resolution. The new dock also includes a cooling fan.

The Switch 2 comes with 256GB of internal storage, and the new Switch 2 Game Cards will load games faster. For digital libraries, however, the Switch 2 will only work with microSD Express cards, which are different from the microSD cards that are compatible with the prior Nintendo Switch. Nintendo will have a Software Transfer feature available to help move games and data from the original Switch to the Switch 2.

The new Joy-Con for the controllers will support mouse controls, and a new C button will be part of a new GameChat communication feature that allows both voice and video chat. There’s also a Nintendo Switch 2 Camera, allowing players to see each other.

The Switch 2 also adds a second USB-C port to the top of the system, which Nintendo says can help connect its new camera accessory or charge the console when playing in tabletop mode. Nintendo also revealed a new Switch 2 Pro Controller with the C button and customizable GL and GR buttons on the back.

Following the presentation, Nintendo unveiled the system’s price of $450 in the US. It will also sell the Switch 2 bundled with a digital version of Mario Kart World for $500.

Mario Kart World

Mario Kart World removes its traditional boundaries and lets drivers roam freely across an entire world of race courses. The game will get its own Nintendo Direct later this month, where we’ll see additional details, but we already know it’ll include traditional races and a Free Roam mode, much like in the Forza Horizon series.

Getting the game bundled for an extra $50 on the cost of the Switch 2 might be the move if you are interested in the game, because Nintendo announced on its website that standalone copies of Mario Kart World will cost $80.

Joy-Con 2 C button and GameChat

Nintendo’s rolling out its new C button across several new Switch 2 controllers. The button will be used for the new GameChat communication features without a headset. The button will be used alongside a microphone on the console itself, which Nintendo says can be used whether it’s docked to a TV or in handheld mode.

In its teaser video, Nintendo promises the microphone will be able to cancel out loud background noises. GameChat will also work with a Nintendo Switch 2 Camera, allowing video chat and various camera-based game modes in supported titles. GameChat will be free at launch through March 31, 2026.

Nintendo Switch 2 Edition games upgrade titles

The Nintendo Switch 2 will play three types of games: original Switch games, Switch 2 games and Switch 2 Edition games that will receive substantial enhancements. For many of these Switch 2 Edition games, you’ll need to buy an upgrade pack if you own the original for Switch. Many of them will get more than just enhanced graphics in the upgrade; for example, Super Mario Party Jamboree will get new games that support the new Joy-Cons’ mouse controls, audio recognition and video camera gameplay options through the Switch 2 Camera.

Other Nintendo Switch games that are getting Switch 2 Edition options include The Legend of Zelda Breath of the Wild, The Legend of Zelda Tears of the Kingdom, Kirby and the Forgotten Land, Metroid Prime 4 Beyond and Pokemon Legends: Z-A. Enhancements vary: The Zelda games will start working with a companion phone app for maps and sending schematics to friends, while Kirby will get a new story that’s exclusive to the Switch 2 Edition. On the third-party side, Civilization 7 will get mouse controls.

Nintendo did not announce what upgrade packs will cost. However, some Switch games will get free updates that will improve performance or enhance features when playing them on the Switch 2. The Switch games getting these updates include:

Hyrule Warriors: Age of Imprisonment

Hyrule Warriors: Age of Imprisonment is a new game set in the world of Zelda that tells the story that leads into The Legend of Zelda: Tears of the Kingdom. This appears to be similar to how Hyrule Warriors: Age of Calamity told the story of a war that led to the events of Breath of the Wild. The teaser shows Zelda discovering that she’s arrived in the past of Hyrule, and the game will expand on how she gets involved in the corresponding Imprisonment War.

Nintendo Switch 2 games

Several other first- and third-party games were spotlighted during the Switch 2 Direct. These include Donkey Bananza, one of the first 3D platforming games featuring DK since 1999’s Donkey Kong 64. Kirby will also return to the racing genre in Kirby Air Riders, which comes more than 20 years after the GameCube racer Kirby’s Air Ride. DragXDrive will use mouse controls to control a futuristic wheelchair basketball game in which players will simulate push and pull motions to control their character.

An onslaught of Switch 2 third-party games were quickly shuffled through during the Direct, which I list below. An unnamed James Bond game is in development at Hitman studio IO Interactive, as is a darker title from Elden Ring creator FromSoftware called The Duskbloods, which will be exclusive to the Switch 2.

The full list of announced Nintendo Switch 2 games includes:

Borderlands 4
Bravely Default Flying Fairy HD Remaster
Cyberpunk 2077: Ultimate Edition
Daemon X Machina: Titanic Scion
Deltarune
Donkey Kong Bananza
Drag x Drive
EA Sports FC
EA Sports Madden NFL
Elden Ring Tarnished Edition
Enter the Gungeon 2
Fast Fusion
Final Fantasy 7 Remake Intergrade
Fortnite
Hades 2
Hitman World of Assassination — Signature Edition
Hogwarts Legacy
Hollow Knight: Silksong
Hyrule Warriors: Age of Imprisonment
Kirby Air Riders
Kirby and the Forgotten Land — Nintendo Switch 2 Edition and Star-Crossed World
Kunitsu-Gai: Path of the Goddess
Mario Kart World
Metroid Prime 4: Beyond — Nintendo Switch 2 Edition
NBA 2K
Nintendo Switch 2 Welcome Tour
Nobunaga’s Ambition: Awakening Complete Edition
Pokemon Legends: Z-A — Nintendo Switch 2 Edition
Project 007
Puyo Puyo Tetris 2S
Rune Factory: Guardians of Azuma — Nintendo Switch 2 Edition
Sid Meier’s Civilization 7 — Nintendo Switch 2 Edition
Split Fiction
Star Wars Outlaws
Starseeker: Astroneer Expeditions
Street Fighter 6
Super Mario Party Jamboree — Nintendo Switch 2 Edition and Jamboree TV
Survival Kids
The Duskbloods
The Legend of Zelda: Breath of the Wild — Nintendo Switch 2 Edition
The Legend of Zelda: Tears of the Kingdom — Nintendo Switch 2 Edition
Tony Hawk’s Pro Skater 3 and 4
WWE 2K
Yakuza 0 Definitive Edition

Among these titles, you can see the full list of June 5 Switch 2 launch day games here.

Nintendo GameCube library coming to Switch 2

The Nintendo Switch Online game library will add GameCube games to the Switch 2. On launch day, these games will initially include The Legend of Zelda: The Wind Waker, SoulCalibur 2 and F-Zero GX, with each game getting enhanced graphics. Online multiplayer will also be added to certain titles. Super Mario Sunshine, Super Mario Strikers and Luigi’s Mansion are among the games set to arrive later. At launch, Nintendo will also sell a GameCube controller, which will be wireless and include a C button for GameChat.