Technologies

2 Million-Year-Old DNA, the Oldest Ever Recovered, Opens Window to the Past

The prehistoric forest of northern Greenland was home to mastodons, reindeer, hares and an abundance of plant life.

As early as 2006, Eske Willerslev and members of his lab ventured into northern Greenland with a drill, extracting cores of sediment from the Kap København Formation. They were hunting for environmental DNA, or eDNA, in their cores — puzzle pieces that could help paint a picture of the plants and animals present in the region 2 million years ago.

But for the longest time, they came up empty-handed. «Every time we had improvements in terms of DNA extraction or sequencing technology, we’d revisit these samples,» Willerslev, an evolutionary geneticist at the University of Cambridge, said in a press briefing on Tuesday.

No matter what, the researchers failed to get what they were looking for. The run of bad luck saw members of the lab turn to the occult for an explanation; they named their troubles «the curse of the Kap København Formation.»

But with steady improvements in DNA extraction and sequencing technologies, the curse was finally broken.

On Wednesday, the team published the results of their 16-year pursuit of ancient DNA in the journal Nature. They were able to sequence eDNA from 41 sediment samples, collected in 2006, 2012 and 2016 from the Kap København Formation and undisturbed by humans for 2 million years. Their analyses revealed that a lush forest replete with reindeer, hares, mastodons and a wide variety of flora once stood in what is now a dull, gray polar desert.

Willerslev, a pioneering geneticist who has previously recovered eDNA from ice cores and shown it could survive in glaciers, noted that the «breakthrough» relied on expertise, advances in genetic sequencing techniques and bioinformatics.

History in the soil

DNA, which carries the instructions for life, is not a particularly sturdy molecule. The bonds that hold it together are weak and, over time, they break down.

This is why, even though we have an abundance of dinosaur fossils, we don’t have any dinosaur DNA. The beasts died out 66 million years ago, and the DNA would simply not survive that long.

When DNA degrades, the once-long strands of information break apart into smaller and smaller pieces. It becomes almost impossible to piece these fragments back together in the right configuration, especially if they are mixed in with a lot of other DNA from the environment.

Think of DNA like a book. Let’s say Alice in Wonderland. If you have the whole book, you can understand the story. But if you’re missing a few pages, you might not understand where the White Rabbit came from or why Alice ended up at a tea party with the Mad Hatter. If you’re missing lots of pages, you probably can’t even tell what the story was to begin with. Alice? Who’s that? And why is she 10 feet tall?

That’s the problem working with ancient DNA. You might be able to retrieve small fragments of DNA but it is generally too fragmented to be able to tell where it came from — and certainly too fragmented to understand where it came from.

But under certain circumstances, DNA fragments can survive deep time.

«The ‘survival time’ of DNA in the environment is incredibly variable and strongly dependent on the environment itself,» notes Michael Knapp, an ecologist and geneticist at Otago University in New Zealand.

Previously, the oldest DNA ever recovered came from mammoth fossils found in the Siberian permafrost. In a Nature paper in 2021, researchers showed that DNA from the mammoth teeth was, potentially, about 1.6 million years old. The DNA recovered was broken up into small fragments but they weren’t degraded so much they couldn’t be pieced back together. The cold temperature of the permafrost certainly helped with this.

It’s a similar story in the new study.

Willerslev and his collaborators postulate that the long survival time of the DNA in their sediment cores was possible for two reasons. The first is the constant cold temperature of the polar desert. The second is the way the DNA is bound to minerals in the cores, preventing degradation over longer time scales. The idea is that these mineral surfaces prevent enzymes from breaking down the DNA.

Karina Sand, a geochemist at the University of Copenhagen and co-author on the paper, explained that one of the technological leaps that enabled this feat was extracting DNA from clay and quartz minerals. The latter provided an abundance of DNA, but the former was harder to extract good DNA from. Fortunately, that leaves the door open for even older DNA extraction.

«If we can get better at extracting the DNA from the clay minerals, then we think we can go further back in time with DNA,» she said.

The research team was able to extract DNA from the sediment cores and begin to read the surviving fragments. These fragments were then compared to a database of genomes (complete DNA sequences) of modern plants and animals, looking for DNA matches. Over time, they were able to fill the blank pages of history, demonstrating the thriving ecosystem of ancient Greenland.

The ancient forest of Greenland

Two million years ago, Greenland was a different place.

«The Kap København ecosystem, which has no present-day equivalent, existed at considerably higher temperatures than we have today,» noted Mikkel Pederson, a geneticist at the Lundbeck Foundation GeoGenetics Centre, in a press release.

In northern Greenland, average temperatures during this time were likely more than 11 degrees Celsius (around 20 degrees Fahrenheit) higher than they are today. Previous studies at Kap København have shown evidence it was home to a boreal forest, but the eDNA extracted and analyzed in the new study provides a complete reimagining of the area, adding in megafauna and a wide variety of plant life.

The headline mammal DNA found in the cores is undoubtedly the mastodon — which is having a bit of a moment thanks to social media. Some of the eDNA found matched to the Elephantidae family, which includes elephants, mammoths and mastodons. It seems mastodons may have roamed Greenland 2 million years ago, though the researchers note the evidence isn’t extremely strong and is based on relatively weak DNA matches.

The team also found DNA related to reindeer, hares and rabbits, and the subfamily of animals that includes lemmings, voles and muskrats. Notably absent, however, is DNA from carnivores. The researchers suggest this is because of their comparably small biomass in relation to the herbivores. «It’s basically a numbers game,» Willerslev said.

One of the more intriguing DNA finds is of the Atlantic horseshoe crab. The species is no longer found at such northern latitudes, and the authors suggest this may mean Kap København experienced warmer sea surface temperatures 2 million years ago. Previous research has suggested the sea surface was warmer at higher latitudes, and the discovery of horseshoe crab DNA lends further support to this hypothesis.

Warmer temperatures are key. Multiple authors on the paper have reiterated the importance of understanding an ecosystem like this, given the effects of global warming. Two million years ago, the climate was changing and the eDNA shows that Arctic species were living with species that loved much warmer climes. This helps scientists to get an understanding of how nature was adapting to those changes and, within the DNA signatures, there may be clues to ways we could help modern-day fauna and flora survive extreme climatic swings.

One of the significant limitations of studying eDNA is that scientists have to postulate about the kinds of species that were living at the time. Knapp notes closely related ancient species might give you a DNA match but this is «somewhat inaccurate» — it provides an approximation of what existed. We may only be able to assign the DNA at a family or order level, so we can’t know exactly what roamed the boreal forest of Greenland 2 million years ago.

Even so, the recovery of DNA this old opens a new window to the prehistoric Earth, a pathway for scientists and researchers to probe the ecosystems that existed long before humans were around. The team will head to northern Canada to extract cores next year and hope to go even further back in time.

The extraction method may even lend itself to finding DNA in more humid climates across the world, like in Africa and Australia.

«If we can begin to explore ancient DNA in clay grains from Africa, we may be able to gather ground-breaking information about the origin of many different species —perhaps even new knowledge about the first humans and their ancestors,» Willerslev said in a statement.

«The possibilities are endless.»

Technologies

Researchers Discover 18 Popular VPNs Are Connected: Why This Matters

All are owned by 3 separate groups but CNET’s recommended VPNs are not on the list

Virtual private networks are popular ways to keep your online activity private and hide your physical location from your internet service provider and apps. But it’s obviously important to choose a safe and secure VPN.

Three university researchers have discovered that 18 of the most widely used VPNs have shared infrastructures with serious security flaws that could expose customers’ browsing activity and leave their systems vulnerable to corrupted data. These VPNs are among the top 100 most popular on the Google Play Store, comprising more than 700 million downloads.

Read more: Best VPN Service for 2025: Our Top Picks in a Tight Race

The peer-reviewed study by the Privacy Enhancing Technologies Symposium found that these VPNs, despite calling themselves independent businesses, are actually grouped into three separate families of companies.

None of CNET’s recommended VPNs — ExpressVPN, NordVPN, Surfshark, Proton VPN and Mullvad — are on the list. (If you currently don’t have a VPN, here’s why you might want to start using one.)

According to the findings, these are the three groups that contain the 18 VPNs:

Family A: Turbo VPN, Turbo VPN Lite, VPN Monster, VPN Proxy Master, VPN Proxy Master Lite, Robot VPN, Snap VPN and SuperNet VPN
Family B: Global VPN, Inf VPN, Melon VPN, Super Z VPN, Touch VPN, VPN ProMaster, XY VPN and 3X VPN
Family C: X-VPN and Fast Potato VPN

Researchers determined that the VPNs in Family A are shared between three providers linked to Qihoo 360, a firm identified by the US Department of Defense as a Chinese military company. The VPNs in Family B use the same IP addresses from the same hosting company.

Know your VPN’s parent company

It’s a cautionary tale about why it’s important to know who’s behind the VPN you’re using, says CNET senior writer Attila Tomaschek.

«It’s also crucial to know what kinds of data the VPN provider is sharing with its parent company and affiliated entities,» Tomaschek said. «Some of these companies may even be compelled to log customer activity and share it with authorities, depending on the jurisdiction in which they operate.»

Despite the warnings, Tomaschek says it’s not so easy to figure out who controls your VPN. But he says there are measures that customers can take.

«Users can do a few things to help ensure the VPN they’re using is reputable,» Tomaschek says. «Check the privacy policy — specifically for terms like ‘logging,’ ‘data sharing’ or ‘data collection.’ A Google search of the provider can help determine whether the VPN has been involved in questionable activity. Read detailed, unbiased reviews from reputable sources. Be especially wary of signing on with a free VPN, even if it’s listed as a top choice in your app store.»

The PETS researchers examined the most downloaded VPNs on Android, looking for overlaps among business paperwork, web presence and codebase. After identifying code similarities, they were able to group the 18 VPNs into three groups. The study was initially spurred by VPN Pro’s own findings, «Who owns your VPN? 105 VPNs run by just 24 companies.»

CNET’s Tomaschek has advice for anyone who has been using one of these 18 VPNs.

«I’d recommend deleting it from your device immediately,» he said. «If you suspect that any sensitive personal data may have been compromised, it’s a good idea to keep an eye on your credit report and look into services like dark web monitoring or identity theft protection.»

Technologies

Today’s NYT Connections: Sports Edition Hints and Answers for Sept. 6, #348

Here are hints and the answers for the NYT Connections: Sports Edition puzzle for Sept. 6, No. 348.

Looking for the most recent regular Connections answers? Click here for today’s Connections hints, as well as our daily answers and hints for The New York Times Mini Crossword, Wordle and Strands puzzles.

Today’s Connections: Sports Edition was a stumper. But if you play cards, the green group is a fun one for sure. If you’re struggling but still want to solve it, read on for hints and the answers.

Connections: Sports Edition is published by The Athletic, the subscription-based sports journalism site owned by the Times. It doesn’t show up in the NYT Games app but appears in The Athletic’s own app. Or you can play it for free online.

Read more: NYT Connections: Sports Edition Puzzle Comes Out of Beta

Hints for today’s Connections: Sports Edition groups

Here are four hints for the groupings in today’s Connections: Sports Edition puzzle, ranked from the easiest yellow group to the tough (and sometimes bizarre) purple group.

Yellow group hint: Racket time.

Green group hint: Ante up!

Blue group hint: NY signal-callers.

Purple group hint: Coach’s CV.

Answers for today’s Connections: Sports Edition groups

Yellow group: Tennis statistics.

Green group: Poker variants, familiarly.

Blue group: Giants QBs, past and present.

Purple group: Teams coached by Lane Kiffin.

Read more: Wordle Cheat Sheet: Here Are the Most Popular Letters Used in English Words

What are today’s Connections: Sports Edition answers?

The yellow words in today’s Connections

The theme is tennis statistics. The four answers are aces, double faults, unforced errors and winners.

The green words in today’s Connections

The theme is poker variants, familiarly. The four answers are hold ’em, horse, Omaha and stud.

The blue words in today’s Connections

The theme is Giants QBs, past and present. The four answers are Manning, Simms, Tittle and Wilson.

The purple words in today’s Connections

The theme is teams coached by Lane Kiffin. The four answers are Mississippi, Raiders, Tennessee and USC.

Technologies

Hollow Knight Silksong Guide: Read These 11 Tips Before Starting

Need help finding rosaries and maps? Here are 11 tips to help you begin your journey.

The indie gaming classic Hollow Knight’s long-awaited sequel, Silksong, is here. Just like the 2019 original, the new game is mysterious and labyrinthine. I’m having an absolute blast playing it, but there are a lot of quirks and challenges you’re going to have to face.

Here are 11 tips to help you navigate the first couple hours of the exciting — and difficult — Silksong.

Read more: I Played Silksong on the ROG Xbox Ally X and I’m Ready for More

11 Hollow Knight: Silksong tips for beginners

Early rosary cache

Rosaries are this game’s version of currency, which you can use to buy amulets and maps from early game merchants. If you die, you’ll have to recover them from your corpse in order to collect what was lost. In the opening area of the game, you can find a rosary cache to give your wallet a boost early on. Right before entering Bone Bottom, there’s a hole to drop down. Hold left as you fall and you’ll find a bone wall you can break. Here you’ll find your reward.

Learn the pogo

Bouncing off enemies is an early way to double jump. To do it, hold down while attacking in the air. It’s much harder to pull off than it was in Hollow Knight due to Hornet’s attacks, which are angled diagonally rather than straight down. However, if Silksong is anything like the first, you’ll be expected to master this technique in order to find and unlock everything in the game — there could be secret areas that can only be reached or bosses that can only be defeated with this technique. Make sure to master this skill early on.

First spool fragment and shortcut

After meeting Shakra, the map merchant, head left into the next screen. There is a flying enemy near the far left wall. Use the pogo jump technique off of them to reach and exit the room in the top left corner. If you kill the enemy, simply leave the area and rest at the nearby bench to respawn them (benches also save your game). After reaching the next room, drop down the first hole. There’s also a rosary cache to your right as you descend. Continue down until you reach a lever, which activates the elevator, giving you a shortcut back to Bone Bottom.

From the top of the elevator shaft, head right. You’ll also see an enemy glowing green below you as you progress. It holds a Moss Berry, and in order to get it, you’ll either have to keep pogo attacking them or fall and slash repeatedly. You can use your new elevator to keep ascending back up if you fall down.

Eventually, you’ll find another cache of rosaries hanging in a small room, but be careful, as they’re boobytrapped with spikes right underneath. However, these spikes only activate once, so you can just wait for them to disappear. Attack the wall in the bottom left of this room to open a secret door that leads to the Spool Fragment — you’ll want to prioritize collecting these, as they increase your max silk capacity.

Buy the first four items from Shakra

Maps are extremely useful in Silksong, so buy them whenever you have the chance. When you first meet Shakra, they’ll have two for you. In addition, you can also buy the Quill and Compass. These will help fill in the map and, if equipped, show your location. Try and grind out enough cash to buy all four of these items. If you’re low on funds, the enemies in the area to your left will drop around five rosaries each, and there’s a nearby bench you can sit on to keep respawning them.

If you followed my advice and collected the Spool Fragment above, you should have more than enough currency to buy all four.

View the entire map

Holding down LB (on Xbox) will bring up the map of your current area. In order to see the entire area, and how each section connects to one another, double tap LB instead.

Choral Commandment

You can find a Choral Commandment, which can be given to a certain collector later on, shortly after encountering Shakra. Climb upwards from their position and to the left. Up again past the lever and there is a breakable wall immediately left of you.

Moss Druid

Right after obtaining the Silk Spear technique, head to the bottom right corner of the room and destroy the webs with your new skill. Inside and past the right exit is Moss Druid (and a save bench) who will give you your first side quest of finding three moss berries. If you found one early on, and a second one that I mentioned while going for the Spool Fragment, you can collect a third just below them by dropping down the hole left of the bench and past a hidden wall in the bottom right corner of the room. Finishing this side quest will earn you an achievement.

Easier healing

Unlike in Hollow Knight, you don’t need to hold down the bind button (B on an Xbox controller) to heal. In Silksong, you simply need to tap it once you have enough silk. This will grant you three pips of life back. You can also heal while in the air, so take advantage of it when landing could mean your death.

Different types of equipment

Hollow Knight only had one type of equipment, but Silksong has four. They’re distinguished by colors: white, red, blue and yellow. White is for your spells, such as the Silk Spear, red is for tools, blue holds amulets, and yellow is for additional items, such as the compass. You can only equip one of each at a time (at least for now).

How to defeat the Bell Beast

The first real boss fight of the game is the Bell Beast and it’s located shortly after collecting the Silk Spear skill.

The Beast has three main attacks. The first will see either side of the ground rumble followed by the Beast surfacing and charging toward you. Simply jump over it and do a pogo attack as it passes. The second attack begins like the first, with the ground on the side of the arena rumbling, but this time the Beast will launch into the air and arc over you. It’s very easy to just walk the opposite direction to avoid getting crushed. You can also quickly counter and get in two attacks while it tries to get unstuck from the wall. The last attack will start a little differently — this time the center ground will rumble. The Beast will pop up and launch two bell projectiles horizontally. To dodge, just hop over the bell.

When you’ve whittled the boss’s health down halfway, the Beast will surface and scream, shaking the whole area. This will cause bells to start dropping down from the ceiling while you continue the fight. They’re pretty easy to avoid since they only bounce once before disappearing. Simply hop over them as you continue the pattern of attacking and dodging the Beast’s three attacks.

Unlocks after the Bell Beast

After defeating the Bell Beast you get an achievement and a silk heart. This will regenerate a little bit of silk automatically after every use. After falling in combat, the Bell Beast will also have a change of heart and become your mode of fast travel.

Make sure to revisit Bone Bottom once you’re victorious as you’ll now be able to take on additional side quests from a sign in town.

Hopefully these tips give you a better grasp of the dangerous and complex world as you begin your adventure in Silksong.